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Heterologous expression of M. tuberculosis Rv1169c in M. smegmatis. (A) Ms_Vec and Ms_Rv1169c were grown into an OD600 of 0.8 and then were subjected to PCR amplification to detect Rv1169c gene. (B) The cell lysates were prepared from acetamide-induced Ms_Vec and Ms_Rv1169c were subjected to Western blot to determine the expression of His-tagged Rv1169c protein in M. smegmatis by mouse anti-His antibody.
