Figure 5.

The sensitivity of Ms_Vec and Ms_1169c strains to acid and surface stress. (A) In vitro growth of recombinant Ms_Vec and Ms_Rv1169c after treatment with different pH gradient for 0, 3, 6, and 9 h. The Ms_Vec and Ms_Rv1169c strains were centrifuged, re-suspended to 5 ml MB 7H9 at an OD₆₀₀ of 0.5, 10-fold serial dilutions of Ms_Vec and Ms_Rv1169c were spotted on MB 7H10 containing Hyg. (B) Survival of Ms_Vec and Ms_Rv1169c after exposure to 0.05% SDS. Re-suspended 5 ml recombinant MS_Vec and MS_Rv1169c (OD₆₀₀ = 0.5) were exposed to 0.05% SDS for 1, 2, 3, and 4 h. And then the recombinant strains were plated onto 7H10 plates by serially ten-fold dilution, the bacterial numbers were counted after 3–4 days of cultivation at 37°C. ^* P < 0.05 and ^** P < 0.01.