Figure 6.

Intracellular survival of recombinant Ms_Vec and Ms_Rv1169c within macrophages. PMA-differentiated macrophages were infected with Ms_Vec or Ms_Rv1169c at an MOI of 10. At 6, 24, 48, and 72 h after infection, the macrophages were washed and lysed using 0.01% SDS. Lysates were plated on MB 7H10 medium containing 100 μg/ml Hyg to determine the bacterial number. ^* P < 0.05.