Table 1.

Genomic, phenotypic, and environmental information for Synechococcus strains of putative ecotypes with different depth distributions^a or from different hot spring mats.

Strain	A4	A14	A1-MS	A1-OS
Strain name	65AY6A5	60AY4M2	65AY6Li	JA-3-3Ab
Putative ecotype (PE)	A4	A14	A1	A1
Collection source	Mushroom Spring	Mushroom Spring	Mushroom Spring	Octopus Spring
Isolation date	September 2010	September 2010	September 2010	July 2002
Isolation temperature (°C)	65	60	65	58–65b
PE relative abundance ≥5% at 60°C Mushroom Spring	400–800 μm	400–960 μm	0–960 μm	0–960 μm
PE relative abundance ≥10% at 60°C Mushroom Spring	640–720 μm	560–960 μm	0–800 μm	0–800 μm
Light adaptation	Low-light	Low-light	High-light	High-light
Sequencing method	Illumina	Illumina	Illumina	Sanger
Depth of coverage	22×	35×	16×	2×
Length (Mbp)	2.98	3.16	2.93	2.93
Number of contigs	9	6	2	1
Largest contig (bp)	2,508,234	3,142,301	2,795,989	2,932,766
%GC content	60.4	60.4	60.3	60.2
CDS	2622	2597	2623	2760
tRNAs	47	47	49c	47
rRNA operons	2	2	2	2
Number of genes unique to the strain with respect to the reference A1-OS	173	204	131	N/A

^aDistributions are expressed as a range of depths where the PE has relative abundance of either ≥5 or ≥10% abundance, rather than emphasizing the peak population abundance of a PE (e.g., Table 1 in Nowack et al., 2015).

^bTemperatures in Octopus Spring fluctuate continuously over a 4.5 min cycle (Miller et al., 1998), therefore the 7°C range of the isolation site is given.

^cA1-MS contains a duplication of the 23S rRNA locus in one operon and two adjacent tRNA loci in one operon.