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. 2015 Jun 23;6:630. doi: 10.3389/fmicb.2015.00630

FIGURE 2.

FIGURE 2

The c-di-GMP level is increased in the oprF mutant. (A) Relative pel and psl mRNA expression in H636 (white bars) and H636C (gray bars) relatively to H103 (dashed black line). (B) c-di-GMP level evaluation using the PcdrA-gfp reporter fusion (full colored bars), and by LC/MS/MS quantification (dotted colored bars), harbored by H103 (black bar), H636 oprF mutant (white bar) and the complemented H636C strain (gray bar). (C) Exopolysaccharide production by H636 harboring pJN105 (dotted white bar) or pJN2133 (gray bar), using the CR release assay. 100% correspond to the EPS production by P. aeruginosa H103. (D) Biofilms formed by H636 harboring pJN105 (dotted white bar) or pJN2133 (gry bar) were quantified relatively to H103 by CV staining. (E) qRT-PCR assays of pelB mRNA level in H636 harboring pJN105 (dotted white bars) or pJN2133 (gray bars), relatively to H103. Each experiment (A–E) was performed at least three times. Statistics were achieved by unpaired t-test: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, NS, not significant, relatively to H103.