FIGURE 3.

The lack of OprF led to increase rsmZ, but not rsmY expression. (A) Relative expression of the small RNAs rsmZ and rsmY in H636 mutant (white bars) and the complemented strain H636C (gray bars), relatively to H103 (dashed black line). (B) Relative rsmZ and rsmY sRNA levels in H636 mutant strain harboring pJN105 (dotted white bars) or pJN2133 (gray bars), relatively to H103 (dashed black line). (C) Western blot analyses of T3SS α-PcrV in P. aeruginosa H103, H636, and H636 harboring pJN2133. Each experiment was performed at least three times. Statistics were done by unpaired t-test. ^∗p < 0.05, ^∗∗p < 0.01, NS, not significant.