Table 1.
Bacterial strains and plasmids used in this study.
| Strains/plasmids | Relevant characteristic(s) | Source |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| DH5α | Cloning host | Life technology |
| SM10pir | Host strain for mini-CTX1 | de Lorenzo and Timmis (1994) |
| Pseudomonas aeruginosa | ||
| H103 | PAO1 derivative | Hancock and Carey (1979) |
| H636 | PAO1 H103 oprF::Ω | Woodruff and Hancock (1988) |
| H636O | pRW5 containing H636 strain | Woodruff and Hancock (1988) |
| H636C | H636 complemented strain with | This study |
| a copy of oprF downstream | ||
| pBAD promoter | ||
| Plasmids | ||
| pRW5 | OprF cloned in pUCP19 vector, Cbr | Woodruff and Hancock (1988) |
| pJN105 | araC-pBAD cassette cloned in pBBR1MCS-5, | Newman and Fuqua (1999) |
| Gmr | ||
| pJN2133 | PA2133 cloned in pJN105, Gmr | Hickman et al. (2005) |
| mini-CTX1 | Plasmid for the integration of genes into | Hoang et al. (2000) |
| the att site of the P. aeruginosa chromosome, Tcr | ||
| mini-CTX- | araC-pBAD cassette cloned in mini-CTX1, Tcr | This study |
| araC-pBAD | ||
| mini-CTX- | oprF cloned upstream pBAD promoter in | |
| araC-pBAD-oprF | mini-CTX-araC-pBAD, Tcr | This study |
| PcdrA-gfp | Rybtke et al. (2012) |
Cbr, carbenicillin resistance; Gmr, gentamycine resistance; Tcr, tetracycline resistance.