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. 2015 Jun 9;2015:154164. doi: 10.1155/2015/154164

Figure 5.

Figure 5

Effect of lowbush blueberry proanthocyanidins (Pcys) (50 μg/mL) on the density of death receptors present at the cell surface of SW620 (a) and SW480 (b) cells. TRAIL-R1 receptor is specifically recognized by a monoclonal antibody Ac1 coupled to Alexa 488 (which emits at 525 nm) whereas TRAIL-R2 receptor is specifically recognized by a monoclonal antibody Ac2 coupled to phycoerythrin (which emits at 575 nm). Fas receptor is specifically recognized by a monoclonal antibody Ac3 coupled to phycoerythrin-Cy5 (PE-Cy5) (which emits at 670 nm). TRAIL-R1 receptor presence is materialized by the mean green fluorescence emitted by Ac1 antibody on SW620 or SW480 cell lines, while TRAIL-R2 receptor is materialized by the mean yellow fluorescence emitted by Ac2 antibody and Fas receptor is materialized by the red green fluorescence emitted by Ac3 antibody. Statistical significant differences (n = 3 independent experiments) based on mean fluorescence (AU) of the cell population between labeled cells in absence of proanthocyanidins and cells labeled and treated with Pcys are represented by the symbol “∗.”