Figure 2.

MSE and RSV attenuate collagen decline in skin tissues of Sod1 ^−/− mice. (a) Sirius red staining of the back skin of Sod1 ^−/− and Sod1 ^+/+ mice treated with the MSE or RSV. Relative mRNA expression of (b) Sirt1, (c) Col1a1, and (d) p53. Each of the mRNA expressions was determined by qRT-PCR (n = 8–12). The statistical evaluations were performed using the two-tailed Student's t-test for unpaired values. These data indicate the mean ± SD; ^∗ P < 0.05, ^∗∗ P < 0.01. The scale bar represents 100 μm.