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. 2015 Jun 23;3:89. doi: 10.3389/fbioe.2015.00089

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Copyright © 2015 Koch, Hammer, Ossmann, Schierle, Sack, Hofmann, Wecks and Boldt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemical DAB-staining of decellular CDI crosslinked implants for MMP3 and TIMP1 in comparison to chemical untreated scaffolds after 1, 9, and 30 days in vivo (n = 6/day/group). Nine days post-implantation, the rate of MMP3 [untreated: (A), CDI: (B)] reactivity increased compared to post-operative day 1. TIMP1 reactivity could not be detected in both untreated (C) and crosslinked (D) scaffolds.