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Immunohistochemical DAB-staining of decellular CDI crosslinked implants for MMP3 and TIMP1 in comparison to chemical untreated scaffolds after 1, 9, and 30 days in vivo (n = 6/day/group). At day 30 post implantation, MMP3 could be detected in both groups [untreated: (A), CDI: (B)], whereas TIMP1 was not detectable in both untreated (C) and CDI crosslinked (D) scaffolds. Bar = 100 μm.
