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. 2015 Jun 23;3:89. doi: 10.3389/fbioe.2015.00089

Figure 4.

Figure 4

Reseeding of crosslinked or chemical untreated ureteral scaffolds with LS48 (A), 3T3 (B), aco2 (C), and smooth muscle cells (D). Cells are able to grow on chemically untreated and crosslinked scaffolds after incubation for 2 weeks. Effects of crosslinking on relative amount of cells could not be detected under seeding with LS48 [(A), n = 9] and Caco2 [(C), n = 9] cells. However, 3T3 cells show an increased infiltrating rate into CDI and GP crosslinked scaffolds, compared to the untreated group [(B), n = 9]. Furthermore, crosslinking with GP caused an increase of smooth muscle cells, grown on surface and infiltrate scaffolds [(D), n = 7]. Data were calculated based on the scoring by two blinded observers: 1 = no cells; 2 = in total <20 cells; 3 = partially 1 cell layer, in total <50 cells, 4 = 1 cell layer around the scaffold; 5 = cells are arranged in multilayer, with matrix-infiltration. Untreated, untreated decellular ureteral tissue; GP, genipin; GA, glutaraldehyde; CDI, carbodiimide. *P < 0.05, **P < 0.01, ***P < 0.001 vs. decellular ureteral scaffolds.