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. 2015 Jun 9;2015:958217. doi: 10.1155/2015/958217

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Copyright © 2015 Hao Han et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Effects of FO+ALA-PS on mRNA and protein expressions of MCP-1 (a-b), IL-1β (c-d), and IL-6 (e-f) in circulating monocytes of mice. After supplementing flaxseed oil with ALA-PS for 18 weeks, total RNA was extracted from circulating monocytes of mice by Trizol. MCP-1, IL-1β, and IL-6 mRNA expressions were analyzed by real-time RT-PCR. The mRNA of β-actin was quantified as an endogenous control. Monocytes lysates were prepared and immunoblotted with corresponding antibody, respectively. Blotting with anti-β-actin was used as a protein loading control. MCP-1, IL-1β, and IL-6 are presented as fold change relative to control. Representative immunoblots are shown. Values are given as mean ± standard deviation of the mean (n = 3). ^a P < 0.05 versus the control; ^b P < 0.05 versus the HFD group; ^c P < 0.05 versus the FO group.