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. 2015 Jun 9;2015:673847. doi: 10.1155/2015/673847

Table 1.

Sequence of primer sets used for quantitative real-time.

Gene symbol Accession number Primer sequences Product size (bp) Efficiency R 2 Reference
IL-8 NM_213867 F 5′-AGAGGTCTGCCTGGACCCCA-3′ 126 1.972 0.999 [28]
R 5′-GGGAGCCACGGAGAATGGGT-3′

IL-6 NM_214399 F 5′-TTCACCTCTCCGGACAAAAC-3′ 122 1.970 0.995 [29]
R 5′-TCTGCCAGTACCTCCTTGCT-3′

TNF-α NM_214022 F 5′-TTCCAGCTGGCCCCTTGAGC-3′ 146 1.873 0.982 [30]
R 5′-GAGGGCATTGGCATACCCAC-3′

COX-2 NM_214321 F 5′-AGAAGCGAGGACCAGCTTTC-3′ 215 1.905 0.981 NCBI/Primer-Blast
R 5′-AAAGCGGAGGTGTTCAGGAG-3′

Hsp70 NM_001123127 F 5′-GCCCTGAATCCGCAGAATA-3′ 152 2.0 0.993 [31]
R 5′-TCCCCACGGTAGGAAACG-3′

CycA NM_214353 F 5′-GCGTCTCCTTCGAGCTGTT-3′ 160 1.907 0.998 [30]
R 5′-CCATTATGGCGTGTGAAGTC-3′

HPRT NM_001032376 F 5′-GGACTTGAATCATGTTTGTG-3′ 91 1.963 0.997 [32]
R 5′-CAGATGTTTCCAAACTCAAC-3′