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. Author manuscript; available in PMC: 2016 May 27.
Published in final edited form as: Dig Dis. 2015 May 27;33(3):351–356. doi: 10.1159/000371687

Figure 2. MF consumption shifts bile composition toward greater proportion of taurocholate and promotes growth of B. wadsworthia (Bw) in vitro.

Figure 2

A. Chemical structures of taurocholic and glycocholic acids, illustrating the distinctive sulfur moiety with taurine conjugation. b. Gall bladder aspirates from SPF IL10−/− mice consuming LF, PUFA or MF for 5 weeks were analyzed by HPLC and mass spectrometry to determine bile composition. c. The gallbladder aspirates were then added to the growth media of Bw at time=0. Normal Bw growth media contains taurine, however added taurine in this media was removed. Bile was added at 1% of media and optical density was recorded every hour for 24 hrs. At 4 hours post-innoculation, Bw growth began to rapidly increased in the media containing bile from MF-fed mice.