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Time courses for the hydrolysis of 90 μM racemic substrate 6 using wild-type PTE and Pmi1525. (A) The reaction was initiated with the addition of 0.5 nM wild-type PTE. After 23 minutes, 50 nM of Pmi1525 was added to hydrolyze the remaining material. (B) Hydrolysis of 90 μM racemic 6 using 1.0 nM wild-type PTE. (C) Hydrolysis of 90 μM racemic 6 using 50 nM Pmi1525.
