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. Author manuscript; available in PMC: 2015 Jun 23.
Published in final edited form as: Hepatology. 2008 Dec;48(6):2027–2039. doi: 10.1002/hep.22592

Figure 2. AA Induces Oxidant Stress Mostly in KCethanol.

Figure 2

KCcontrol and KCethanol were incubated with 0-10μM AA for 24h. Intracellular lipid peroxidation assessed by cis-parinaric acid fluorescence (A). Lipid peroxidation end products secreted to the culture medium are shown as nmols of TBARS (B). Intracellular hydroperoxides (mostly H2O2) were measured by flow cytometry using DCFDA (C). Levels of hydroperoxides (mainly H2O2) released to the culture medium were evaluated by the FOX method (D). Intracellular O2.− was determined by flow cytometry using DHE (E). In all panels, results are expressed as means ± SEM of N>3. *P<0.05, **P<0.01, and ***P<0.001 for AA-treated vs. non-treated, and •P<0.05, ••P<0.01, and •••P<0.001 for ethanol vs. control.