Fig. 5.

Validation of expression of HPSE2 and MMP11 using RT-qPCR. Seven samples were picked from the training set, representing Luminal A (C427 and C696), Luminal B (C927 and C369), Triple negative (C430 and C434), and normal tissue (N518) respectively. Fold changes of gene expression were calculated with the 2^-ΔΔC_T method, using β-actin as the house keeping gene. Results were shown as mean ± SEM from triplicates (n = 3). *p < 0.05 compared with N518, **p < 0.001 compared with N518