Cases 5 (A, C, E) and 6 (B, D, F). A World Health Organization (WHO) grade III anaplastic oligoastrocytoma of the right frontotemporal lobe (A) was immunonegative for R132H IDH1 (C), even though it showed an R132H IDH1 mutation on sequencing (E). In another case, a 36-year-old man had a right temporal WHO grade III anaplastic astrocytoma (B) that, aside from false-positive staining in red blood cells (D, arrow), was negative for R132H IDH1 on immunohistochemistry. Sequencing revealed an uncommon R132S IDH1 mutation (F).