Fig. 2.

EGFP-expressing P. agglomerans persisted inside the foregut of the glassy-winged sharpshooter. (a) GWSS were fed EGFP-expressing P. agglomerans via an artificial feeding system for 48 h. The insects were moved to naive grape plant for 24 h to wash out unattached bacteria. They were subsequently housed on another naive grape plant for up to 15 days. Insects were collected daily, and processed as described. Homogenates were plated on LB plates to determine the presence of P. agglomerans. (b) Percent of GWSS carrying EGFP-expressing P. agglomerans over a period of 15 days. No difference in proportions of GWSS carrying EGFP-expressing P. agglomerans was observed. p = 0.1979 by chi-squared test for homogeneity