Table 2.
List of the primary antibodies and dilutions used for western blotting/ immunoprecipitation (WB/IP) and immunostaining (ICC)
| Antigen | Antibody | Species | Company | WB/IP | ICC |
|---|---|---|---|---|---|
| V5 | monoclonal | mouse sv5-pk | Santa Cruz | 1:500/IP(2 μg) | 1:300 |
| V5 | polyclonal | rabbit | Genetex | 1:5000 | 1:200 |
| myc | monoclonal | mouse 9E10 | Santa Cruz | 1:1000/IP(2 μg) | 1:200 |
| flag | monoclonal | mouse M2 | Agilent Tech | 1:2000/IP(2 μg) | |
| Cox4 | polyclonal | rabbit | Genetex | 1:1000 | |
| Tom20 | polyclonal | rabbit | Santa Cruz | 1:500 | |
| Gsdma3 | polyclonal | rabbit | Home made | 1:500 | 1:200 |
| β-actin | polyclonal | rabbit | Genetex | 1:5000 | |
| GAPDH | Polyclonal | rabbit | Genetex | 1:5000 | |
| Hsp90α/β | polyclonal | rabbit | Santa Cruz | IP (2 ug) | |
| Hsp90β | polyclonal | rabbit | Genetex | 1:2000 | |
| Hsp70 1A | polyclonal | polyclonal rabbit | Genetex | 1:2000 | |
| STIP1/Hop | polyclonal | rabbit | Genetex | 1:2000 | |
| TRAP1 | monoclonal | mouse | Genetex | IP (2 ug) | |
| TRAP1 | polyclonal | rabbit | Genetex | 1:4000 | |
| AKT1 | polyclonal | rabbit | Millipore | 1:2500 | |
| Tom70 | monoclonal | mouse | Santa Cruz | 1:2000/IP(2 ug) |