Figure 4. Activation of ANO6 current and PLS requires high intracellular Ca²⁺ concentrations.

(A) Average current–voltage relationships of currents recorded ∼20 min after establishing whole-cell recording in Ano6-expressing cells patched with 20 μM (black squares, N = 6) or 200 μM Ca²⁺ (red circles, N = 10) in the patch pipet. (B) Annexin-V binding in Ano6-expressing cells patched with 20 μM (black squares, N = 5) and 200 μM (red circles, N = 15) Ca²⁺ in the patch pipet. Error bars are S.E.M.

DOI: http://dx.doi.org/10.7554/eLife.06901.006