Table I.
Untreated | 0.29 ± 0.02a,b |
LA | 0.14 ± 0.06a |
DHA | 0.22 ± 0.04a |
Butyrate | 0.62 ± 0.04c |
Butyrate + LA | 0.44 ± 0.06b |
Butyrate + DHA | 0.74 ± 0.08c |
Cultures were incubated with 5 mM butyrate and 50 μM DHA, LA or no fatty acid treatment as described in the Materials and methods. Apoptosis was measured by DNA fragmentation ELISA. Data represent mean absorbance at 405 nm ± SE divided by the total number of adherent cells per dish, n = 4-6 separate wells from 1 of 4 representative experiments. Values not sharing the same letters are significantly different (P < 0.05).