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. Author manuscript; available in PMC: 2015 Jun 23.
Published in final edited form as: Biotechniques. 2013 May;54(5):257–264. doi: 10.2144/000114028

Figure 4. Quality control of NAPPA microarrays with 849 TB genes.

Figure 4

Expression clones encoding the target proteins fused to a C-terminal GST tag were printed along with a polyclonal anti-GST antibody in duplicate on the array surface. DNA capture was confirmed by PicoGreen (PG) staining (DNA, left), in situ protein expression and capture were assessed by GST detection using a monoclonal antibody (protein, middle). The correlation of duplicate spots in one slide (upper right) and between two different slides (lower right) is 0.96 and 0.81, respectively (GST color code: red>orange>yellow>green>blue).