Table 2.
Comparison of the performance of multiplexed antibody assay without and with MiNC
| Name | LOD (ng/ml) | Dynamic range (ng/ml) | Intra-CV(%)* | Inter-CV(%)* | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| MiNC | MiNC | MiNC | MiNC | |||||||
| − | + | − | + | − | + | p-value | − | + | p-value | |
| CYRAB | 0.4 | 0.4 | 0.4 – 2000 | 0.4 – 2000 | 9 | 3 | <0.001 | 15 | 10 | 0.278 |
| c-Jun | 57.2 | 22.2 | 57.2 – 40000 | 22.2 – 40000 | 9 | 3 | <0.001 | 6 | 10 | 0.235 |
| PRDX4-3 | 5.6 | 3.4 | 5.6 – 20000 | 3.4 – 20000 | 12 | 4 | <0.001 | 10 | 10 | 0.944 |
| AV | 10 | 4 | 10 | 10 | ||||||
*
The intra-CV and inter-CV were calculated as the average CV across eight antibody concentrations.