Figure 3.

Comparisons of the effect of UAA incorporation at different sites on expression of VSVg protein and propagation/infectivity of progeny vectors. The expression levels of VSVg, which reflect the efficiency of UAA incorporation, were analyzed by western blotting (Supplementary Figure S2), quantitated by scanning the VSVg band and then normalized to that of wild-type VSVg. The infectivity of lentiviral vectors containing NAEK was detected by luciferase assay and normalized to that of the wild-type lentiviral vector. All quantitative data shown are average values with standard deviations from triplicate experiments.