Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 May 4;43(11):5572–5585. doi: 10.1093/nar/gkv414

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 1. — (A) Imino proton resonance assignment of the stabilized hsp17^stab RNAT: imino region of a ¹H-¹H-NOESY spectrum (top) and HNN-COSY spectrum (below). Resonances of G2 and C41 originate from the mutation A40CC, which was introduced to stabilize stem I. Sequential walks for stems I and II are highlighted in red (G2-U38) and black arrows (G27-U31). Both experiments were recorded at T = 284 K. (B) Imino resonance assignment of the hsp17^rep RNAT: imino region of a ¹H-¹H-NOESY spectrum (top) and HNN-COSY spectrum (below). Sequential walk is indicated by black arrows (G27-U40). Both experiments were recorded at T = 273 K. Base-pair numbering refers to the wild-type sequence shown in (D), G* denotes the mutation AAC(33–35)G. (C) Imino proton resonance assignment of hsp17 based on comparison of ¹H-spectra of hsp17^rep and hsp17^stab recorded at 274 K. (D) Secondary structure of hsp17, hsp17^rep and hsp17^stab based on NMR resonance assignment. RBS denotes the ribosome binding-site.