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. 2015 May 4;43(11):5572–5585. doi: 10.1093/nar/gkv414

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — (A) Temperature dependence of the fraction unfolded obtained from CD-melting curves of hsp17 (top) and hsp17^rep (bottom). (B) Temperature dependence of Gibbs energies of the base-pair stability. ΔG_diss values (circles) were calculated from the imino exchange rates obtained at catalyst concentrations of 2 and 29 mM HPO₄²⁻ using internal and external transition state parameters. Dashed lines are extrapolations of the Gibbs energy assuming the nucleobase pair is still formed. Grey-shaded area in (A) and (B) highlights the temperature range T_m ± 2.5°C.