Table 2. Fit results of the individual base-pair stabilities in hsp17rep.
hsp17rep | ΔHdiss | ΔΔHdiss | ΔSdiss | ΔΔSdiss | ΔGdiss (T = 20°C) | ΔΔGdiss (T = 20°C) |
---|---|---|---|---|---|---|
[kJ/mol] | [kJ/mol] | [J/(mol K)] | [J/(mol K)] | [kJ/mol] | [kJ/mol] | |
G3/U40 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
U4 | 53.0 | 6.0 | 137.8 | 21.6 | 12.6 | 0.4 |
U38 | 102.1 | 8.1 | 283.0 | 27.5 | 19.2 | 0.2 |
U7/U37 | 86.9 | 8.0 | 206.5 | 26.2 | 26.4 | 0.4 |
G* | 277.8 | 19.2 | 781.2 | 59.3 | 48.8 | 1.9 |
U32 | 70.0 | 4.3 | 148.5 | 14.1 | 26.5 | 0.3 |
U31 | 79.7 | 12.6 | 185.7 | 42.7 | 25.3 | 0.3 |
U11 | 43.0 | 3.6 | 73.8 | 12.2 | 21.3 | 0.2 |
G30 | 45.8 | 4.6 | 82.9 | 15.3 | 21.5 | 0.2 |
U12 | 38.8 | 7.5 | 56.7 | 25.7 | 22.4 | 0.2 |
G28 | 232.5 | 14.4 | 637.5 | 44.4 | 45.6 | 1.4 |
G27 | 95.9 | 4.2 | 232.4 | 13.7 | 27.8 | 0.3 |
U15 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
n.d., not determinable due to low base-pair stability and resultant exchange broadening of the imino resonance. ΔΔHdiss, ΔΔSdiss and ΔΔGdiss (T = 20°C) represent the Monte Carlo error. Nucleobase numbering of hsp17rep is based on hsp17, G* denotes the mutation AAC(33–35)G.