Figure 7.

Contribution of binding sites to regulation of Rv0250c and Rv0249c. (A) The expression of the WWWW construct was compared to each of the individual promoters using ß-galactosidase assays. Significance is shown for individual promoters relative to the WWWW promoter. (B and C) Activity of the mutated dual promoter fragments was assessed in late-log (solid bars) or stationary (striped bars) cultures grown in (B), ambient oxygen or (C) hypoxic (1.3% oxygen + 5% CO₂) conditions. The means with standard deviations are shown from three independent experiments. (*P < 0.05, **P < 0.005 as determined by a two-tailed students t-test).