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. 2015 May 4;43(11):5501–5523. doi: 10.1093/nar/gkv428

Figure 7.

Figure 7.

Generation of DRBD18 knockdown cell lines complemented with untagged DRBD18 variants. (A) Expression of untagged DRBD18 restores cell growth. Green line indicates cells in which neither DRBD18 RNA nor expression of exogenous DRBD18 has been induced. Red line indicates cells in which endogenous DRBD18 is repressed and untagged wild type protein is expressed. (B) DRBD18 RNA and protein levels in cell lines complemented with untagged DRBD18. Top panel shows qRT-PCR analysis of endogenous DRBD18 transcript levels normalized against 18S rRNA. Error bars depict standard deviation from 3–6 determinations. Bottom panel shows anti-DRBD18 western blot analysis of cells expressing ectopic DRBD18 constructs. Complemented cells were harvested 2 days post-induction, lysed in SDS-PAGE sample buffer, and resolved via 10% SDS-PAGE.