Skip to main content
. 2015 May 1;43(11):5409–5422. doi: 10.1093/nar/gkv430

Figure 3.

Figure 3.

HDAC2 blocks reprogramming at the pre-iPS cell stage by impeding the transcriptional activation of maturation phase-related genes. (A) qRT-PCR analyses of Oct4 and the maturation phase-related genes in P1 and P2 clones treated without or with dox for 72 h. (B) Immunostaining for Nanog and Oct4-GFP reporter activity in P1 and P2 clones treated with dox for 36 and 72 h. (C) Phase contrast and fluorescence images of P1 clone transfected with control shRNA and shNanog after dox treatment. Scale bar, 100 μm. Flow cytometry analysis of Oct4-GFP reporter activity (below). (D) qRT-PCR analysis of maturation phase genes (Nanog, Sall4, Esrrb, Rex1, Tcl1 and Cripto) in P1 clone transfected with control shRNA and shNanog after dox treatment. Gapdh was used as an internal control. (E) Representative western blot for HDAC2 and Nanog expression in P1 clone transfected with control shRNA or shNanog upon dox treatment. The data in a and d represent the means ± S. E. M. of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 (two-tailed Student's t-test).