Table 2. Thermal stability of a fully matched duplex AGA/TCT and corresponding duplexes containing an AP site flanked by adenines and with various bases opposite an AP site in the presence of Λ- and Δ-[Fe2L1a3]Cl4.
| Compound | ΔTm (°C) at 1:1a | ΔTm (°C) at 2:1b |
|---|---|---|
| AGA/TCT (Tm = 46.3°C) | ||
| Λ-[Fe2L1a3]Cl4 | 0.2 | 0.8 |
| Δ-[Fe2L1a3]Cl4 | 0.1 | 0.3 |
| AFA/TGT (Tm = 29.1°C) | ||
| Λ-[Fe2L1a3]Cl4 | 11.1 | 11.8 |
| Δ-[Fe2L1a3]Cl4 | 7.1 | 7.8 |
| AFA/TCT (Tm = 25.9°C) | ||
| Λ-[Fe2L1a3]Cl4 | 13.3 | 14.7 |
| Δ-[Fe2L1a3]Cl4 | 12.7 | 13.5 |
| AFA/TAT (Tm = 28.5°C) | ||
| Λ-[Fe2L1a3]Cl4 | 9.8 | 10.8 |
| Δ-[Fe2L1a3]Cl4 | 7.3 | 8.5 |
| AFA/TTT (Tm = 27.6°C) | ||
| Λ-[Fe2L1a3]Cl4 | 10.6 | 11.8 |
| Δ-[Fe2L1a3]Cl4 | 10.1 | 11.2 |
aHelicate:duplex was 1:1.
bHelicate:duplex was 2:1.