Table 3. Thermal stability of a fully matched duplex CGC/GCG and corresponding duplexes containing an AP site flanked by cytosines and with various bases opposite an AP site in the presence of Λ- and Δ-[Fe2L1a3]Cl4.
| Compound | ΔTm (°C) at 1:1a | ΔTm (°C) at 2:1b |
|---|---|---|
| CGC/GCG (Tm = 54.4°C) | ||
| Λ-[Fe2L1a3]Cl4 | 0.3 | 0.7 |
| Δ-[Fe2L1a3]Cl4 | -0.1 | 0.2 |
| CFC/GGG (Tm = 36.8°C) | ||
| Λ-[Fe2L1a3]Cl4 | 9.0 | 9.3 |
| Δ-[Fe2L1a3]Cl4 | 4.8 | 6.4 |
| CFC/GCG (Tm = 31.3°C) | ||
| Λ-[Fe2L1a3]Cl4 | 14.1 | 14.6 |
| Δ-[Fe2L1a3]Cl4 | 11.5 | 12.0 |
| CFC/GAG (Tm = 35.7°C) | ||
| Λ-[Fe2L1a3]Cl4 | 5.8 | 8.5 |
| Δ-[Fe2L1a3]Cl4 | 3.5 | 5.4 |
| CFC/GTG (Tm = 33.0°C) | ||
| Λ-[Fe2L1a3]Cl4 | 12.5 | 13.2 |
| Δ-[Fe2L1a3]Cl4 | 11.2 | 11.9 |
aHelicate:duplex was 1:1.
bHelicate:duplex was 2:1.