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. Author manuscript; available in PMC: 2016 Apr 28.
Published in final edited form as: Cell Rep. 2015 Apr 16;11(4):605–617. doi: 10.1016/j.celrep.2015.03.056

Figure 6. Regulation of Type I IFN responses by ULK1/2 activity.

Figure 6

(A) Ulk1/2+/+ and Ulk1/2−/− MEFs were seeded in quadruplicate in individual wells of 96 well plates, and then treated with mouse IFNα for 16 hours, as indicated. Ulk1/2+/+ cells were subsequently challenged with a 1:2×104 dilution of encephalomyocarditis virus (EMCV), and the Ulk1/2−/− cells with a 1:106 dilution of EMCV. EMCV-induced cytopathic effects (CPE) were determined 24 hours later. The data are expressed as percent protection from CPE adjusted to viral infective dose (% Protection-AVI). Values shown represent means ± SD of two independent experiments. See also Figure S3. (B) U937 cells were transfected with either control siRNA or ULK1 siRNA, and leukemic CFU-L colony formation was assessed in clonogenic assays in methylcellulose in the presence or absence of human IFNβ, as indicated. Data are expressed as percent colony formation of control siRNA-transfected untreated cells, and bar graphs represent means ± SE of five independent experiments. Statistical analysis was performed using Student’s t-test (*p < 0.05).