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. Author manuscript; available in PMC: 2015 Jun 23.
Published in final edited form as: Methods Enzymol. 2015 Feb 27;558:3–37. doi: 10.1016/bs.mie.2015.01.008

Table 10.

Primer labeling reaction

Component Final concentration Stock Amount (μl)
Oligonucleotide 440–660 ng/μl 4–6 mg/ml 11
SE-dye 2.52 μg/μl 18 mg/ml 14
Sodium tetraborate buffer (pH 8.5)a 75 mM 0.1 M 75
a

Prepare freshly before the coupling reaction or keep frozen aliquots at −20 °C.