Table 10.
Component | Final concentration | Stock | Amount (μl) |
---|---|---|---|
Oligonucleotide | 440–660 ng/μl | 4–6 mg/ml | 11 |
SE-dye | 2.52 μg/μl | 18 mg/ml | 14 |
Sodium tetraborate buffer (pH 8.5)a | 75 mM | 0.1 M | 75 |
Prepare freshly before the coupling reaction or keep frozen aliquots at −20 °C.