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. Author manuscript; available in PMC: 2015 Jun 23.
Published in final edited form as: Methods Enzymol. 2015 Feb 27;558:3–37. doi: 10.1016/bs.mie.2015.01.008

Table 15.

Folding reaction

Component Final amount/conc. (+) Reagent sample (μl) (−) Reagent sample (μl)
RNA 20 pmol
5 × monovalent mixture (Table 16) 1× 98 98
MgCl2 250 mM 25 mM 49 49

Add deionized H2O to 490 μl.