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. Author manuscript; available in PMC: 2015 Jun 23.
Published in final edited form as: Methods Enzymol. 2015 Feb 27;558:3–37. doi: 10.1016/bs.mie.2015.01.008

Table 24.

Annealing reaction

Component Final amount/ conc. (+) Reagent sample (μl) (−) Reagent sample (μl)
RNA 1 pmol x x
2 μM labeled primer (JOE) 0.1 μM 1 3
2 μM labeled primer (FAM) 0.1 μM – 1
2 mM Na-EDTA (pH 8.5) 0.1 mM 1 1
5 M betaine 1 M 4 4

Add deionized H2O to 12 μl.