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. Author manuscript; available in PMC: 2015 Jun 23.
Published in final edited form as: Methods Enzymol. 2015 Feb 27;558:3–37. doi: 10.1016/bs.mie.2015.01.008

Table 25.

Reverse transcriptase (RT) mixture

Component Final concentration Stock Amount (μl)
RT buffer 2.5× 5× 96
DTT 12.6 mM 100 mM 24
Betaine 944 mM 5 M 36
dNTP mix 1.26 mM 10 mM 24
Superscript III RT 10 U/μl 200 U/μl 9.6
Deionized H2O 1