Skip to main content
. Author manuscript; available in PMC: 2015 Jun 23.
Published in final edited form as: Methods Enzymol. 2015 Feb 27;558:3–37. doi: 10.1016/bs.mie.2015.01.008

Table 28.

Spectral-calibration PCR reaction

Component Final concentration Stock Amount (μl)
Linearized pUC18 plasmid 0.1 ng/μl 0.2 ng/μl 0.5
Thermo sequenase buffer 1× 10× 4
dNTP mixture 75 μM 0.75 mM 4
Labeled primer 0.0125 pmol/μl 2 μM 5
Thermo sequenase 0.16 U/μl 4 U/μl 1.6

Add deionized H2O to 40 μl.