Table 28.
Component | Final concentration | Stock | Amount (μl) |
---|---|---|---|
Linearized pUC18 plasmid | 0.1 ng/μl | 0.2 ng/μl | 0.5 |
Thermo sequenase buffer | 1× | 10× | 4 |
dNTP mixture | 75 μM | 0.75 mM | 4 |
Labeled primer | 0.0125 pmol/μl | 2 μM | 5 |
Thermo sequenase | 0.16 U/μl | 4 U/μl | 1.6 |
Add deionized H2O to 40 μl.