Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jun 22;209(6):803–812. doi: 10.1083/jcb.201501094

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Haynes et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 2. — Changes in GMFβ expression level alter cell shape and lamellipodial dynamics. (A) Western blot showing GMFβ expression of created cell lines. (B) GMFβ-depleted (KD) and -overexpressing (OE) cells show phenotypic changes versus control (CNTL) and knockdown-rescue (KDR) cell lines. Bar, 50 µm. (C) Cell area quantified from micrographs. (D) Example kymographs for GMFβ KD, CNTL, and GMFβ OE. Bar, 10 µm. (E) Protrusion rate in micrometers per minute measured from kymography. (F) Protrusion distance in micrometers. (G) Protrusion duration in minutes. (H) Retraction rate in micrometers per minute. (I) Mean number of retractions per protrusion. (J) Retraction length in micrometers. For all graphs, error bars represent 10th–90th percentile. Kruskal-Wallis multiple comparison testing was performed, and significance was measured with a Dunn’s post-test. ***, P < 0.001; **, P < 0.01; *, P < 0.05. n ≥ 20.