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. 2015 Jun 22;209(6):843–861. doi: 10.1083/jcb.201412050

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© 2015 Seybold et al.

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Figure 4. — In vivo SPB localization of Kar1 depends on Sfi1. (A) Kar1 levels at the SPB in C-terminally elongated Sfi1 cells. The RFI of yeGFP-Kar1 at the SPBs was measured in the indicated α-factor–arrested cells. (B) Timeline of experiments performed in C and D. (C) Localization of yeGFP-Kar1 upon degradation of dg-SFI1. The percentage of cells showing Kar1 mislocalization at 37°C is provided. (D) As in C, but with Nic96-mCherry as NE marker. n, number of analyzed SPBs per time point. ***, P ≤ 0.001; ns, P > 0.05. Error bars indicate SD. Bars, 5 µm.