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. 2015 Jun 22;209(6):789–801. doi: 10.1083/jcb.201503132

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© 2015 Westhorpe et al.

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Figure 3. — CENP-A assembly on chromatin arrays requires M18BP1 isoform 2. (a, top) M18BP1 levels in egg extract after mock depletion (−), M18BP1 depletion (Δ), or complementation with M18BP1 isoform 1 (bottom band of doublet), isoform 2 (top band of doublet) or both (1, 2, and 1/2, respectively). (bottom) A nonspecific band shown as a loading control. (b) M18BP1 (left) and FLAG–xCENP-A (right) levels on chromatin beads after M18BP1 depletion from egg extracts as described in a. All graphs show the means ± SEM, normalized to the mock-depleted CENP-A signal, n = 4. (c) CENP-C levels on chromatin beads after M18BP1 depletion as described in a.