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. 2015 Jun 22;209(6):789–801. doi: 10.1083/jcb.201503132

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© 2015 Westhorpe et al.

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Figure 5. — FLAG–xCENP-A assembly requires the CATD and CAC. (a) Fluorescence images of FLAG–xCENP-A assembly on low saturation chimeric chromatin. Myc-H4, FLAG–xCENP-A, and bead autofluorescence are shown. (b) Quantification of FLAG–xCENP-A assembly as shown in a; all bars represent means ± SEM normalized to the signal on CENP-A arrays; n = 4. (c) FLAG–xCENP-A assembly on high saturation CENP-A/H3 chimeric chromatin. Normalized as in b. n = 4. (d) Fluorescence images of FLAG–xCENP-A assembly on high saturation CENP-A/H3 chimeric chromatin. Labeled as in a. (e) Protein levels of CENP-C, FLAG–xCENP-A, Myc-H3, hCENP-A, and H3 with son saturated chromatin arrays containing 80% hCENP-A nucleosomes and 20% Myc-H3 nucleosomes after incubation in extract supplemented with buffer (−) or with FLAG_xCENP-A and xHJURP RNA (+). Bars, 5 µm.