Table 1. Quantitative features of MFA2 mRNA granules in αF-treated WT cells.
| Type of mRNA | Intensity | CHX sensitivity | Intensity mean (IOD)a | Approximate | Velocityb | Type of motility |
|---|---|---|---|---|---|---|
| % | µm/s | |||||
| MFA2 mRNA | High | + | 8,000–10,000 | 12 | 0.05 ± 0.04 | Vibrational |
| Low subgroup 1 | − | 1,800–2,200 | 27 | 0.04 ± 0.03 | Vibrational | |
| Low subgroup 2 | − | 800–1,100 | 16 | 1.81 ± 0.78 | Translocational | |
| Low subgroup 3 | − | 800–2,200 | 45 | 0.45 ± 0.09 | Oscillatory with slow translocation | |
| PGK1 mRNA | Low subgroup 1 | − | 1,400–2,250 | 25 | 0.09 ± 0.08 | Vibrational |
| Low subgroup 2 | − | 500–1,200 | 75 | 0.79 ± 0.06 | Oscillatory with slow translocation |
Three independent videos were taken, using time-lapse technology with a Fast Acquisition AxioVision microscope. Videos were analyses of between 25 and 30 frames, each between 73 and 113 ms, without intervals between shots. These videos were used to determine IOD, granule velocity, and granule number per cell.
IOD refers to integrated optical density (IOD) of the fluorescent granules, obtained using the ImageJ program. For determining IOD, n = 100 random snapshots, each one contains several cells (5–9).
Tracking of individual granule was performed using Imaris program version 7.1 (Bitplane AG). Individual granules were tracked throughout the video, unless the granule appeared during a portion of the video (at least in eight consecutive frames). Average values and SD were calculated based on 10–15 granules for each group/subgroup.