Table 5. Plasmids used in the study.
| Plasmidsa | Genotype | Yeast marker | Source |
|---|---|---|---|
| pMC314b | GPDp-U1A-GFP | LEU2 | P. Silverc; Brodsky and Silver, 2002 |
| pMC313 | GPDp-MFA2-U1Ax16-3′UTR of MFA2 | URA3 | This work |
| pSA01 | GPDp-MFA2-mCHERRY- U1Ax16-3′UTR of MFA2 | URA3 | This work |
| pMC409 | PGK1p-PGK1-U1Ax16-3′UTR of PGK1 | URA3 | R. Parker; Sheth and Parker, 2003 |
| pMC414 | DCP2p-DCP2-RFP | HIS3 | This work |
| pMC413 | LSM1p-LSM1-RFP | HIS3 | This work |
| pMC389 | PAB1p-PAB1-RFP | HIS3 | This work |
| pMCB470 | EDC3p-EDC3-mCh | TRP1 | R. Parker; Buchan et al., 2008 |
| pMC522 | MET25p-MS2-mCh | HIS3 | D. Ravehd; Lavut and Raveh, 2012 |
| pMC475b | GPDp-MFA2-MS2 × 2 | URA3 | R. Parker; Sheth and Parker, 2003 |
a
All plasmids, unless otherwise indicated, contain CEN and therefore are present in one or two copies per cell.
b
Multicopy 2μ plasmid.
c
Harvard Medical School, Boston, MA.
d
Ben-Gurion University of Negev, Beer-Sheva, Israel.