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. 2015 Jun 23;10(6):e0129481. doi: 10.1371/journal.pone.0129481

Fig 4. Resilience of mitochondrial function in response to pyruvate after A1254 exposure in SH-SY5Y cells.

Fig 4

Panel A. OCR measurement after 6 hrs exposure to A1254 (5 μg/ml) in the presence or absence of pyruvic acid. Basal OCR, ATP-linked and maximal respiration were measured by the injection of oligomycin (5 μM), FCCP (1.5 μM) and antimycin (1 μM) plus rotenone (1 μM) at indicated times. Panel B. ECAR measurement after 6 hrs exposure to A1254 (5 μg/ml) in the presence or absence of pyruvic acid. Basal ECAR, maximal ECAR, glycolitic capacity and glycolytic reserve were measured by injection of glucose (10 mM), oligomycin (5 μM) and 2-DG (100 mM) at the time indicated. Data = mean±SE. Data were normalized to number of cells.