Fig 5. Deletion of IKKβ is associated with replating and repopulation advantage.
A) Equal number of wild type, IKKβΔ/Δ or p65Δ/Δ bone marrow cells were plated and myeloid CFUs were enumerated and replated every 7 days. The average number of colonies at the indicated replating generation is shown. B, C) Lethally irradiated CD45.1 mice were injected with 5E5 CD45.2 marrow cells from wild type or IKKβΔ/Δ along with 5E5 CD45.1 marrow cells. Engraftment of bone marrow was assessed using FACS analysis for CD45.1 and CD45.2. Representative marrow FACS plots and the average proportion of CD45.2 engraftment in the marrow 20 weeks after transplant is shown (n = 5).