Fig 6. Deletion of IKKβ is associated with altered expression of hematopoietic transcription factors.
Total cellular RNA was isolated from Lin- marrow cells of mice with the indicated genotypes and the expression of A) myeloid differentiation associated genes, B) hematopoietic transcription regulators C) transcription factors regulating erythropoiesis, and D) anti-apoptotic Bcl2 family members was analyzed using qRT-PCR. Average relative expression from 3–4 independent experiments is shown. E) LSK cells were isolated by FACS from wild type or IKKβΔ/Δ mice, RNA was extracted and subjected to qRT-PCR. *-denote p<0.01. **denotes p<0.001. F) Cell lysates were obtained from wild-type (WT) or IKKβΔ/Δ Lin- bone marrow cells and subjected to Western blot analysis with the indicated antibodies. Representative gels with relative band intensity values are shown.